Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 08/24/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | The School for Science and Math at Vanderbilt |
| Observations | Was found in my moms rose bush |
| Putative identification | Arthropoda Hexapoda |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The wolbachia positive controls were the only ones that showed in the electrophoresis process for Wspec |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
GTTCTTTAATTGGAGATGATCAAATTTATAATGTTATTGTTACAGCTCACGCTTTTATTATAATTTTTTTTATAGTTATA CCCATTATAATTGGTGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCCCCAGATATAGCTTTTCCACGAATAAA TAATATAAGATTTTGACTTCTCCCACCCTCAATTTTATTATTATTAATTAGAAGAACAGTAGAAAGAGGAGCAGGAACTG GATGAACTGTATACCCCCCTTTATCAGCAAGAATTGCTCATGCAGGACCAGCAGTAGATTTAACAATTTTTTCTCTTCAT CTTGCAGGTATATCTAGAATTATAGGTGCTGTAAACTTTATTACAACTATAATTAATATAAAACCACTTTATATAAATCA AACCCAAGTACCTCTTTTTGTATGATCAGTAGGAATTACAGCTCTATTATTATTACTTTCATTACCAGTACTTGCAGGAG CAATTACTATACTCTTAACAGATCGAAATCTTAATACTTCATTCTTTGATC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hexapoda was found to be negative for Wolbachia. |
Water Boatman