Wolbachia DNA Extraction Praying Mantis

September 1, 2021

Sample information
Picture
Photos by: Nathalie G.
Location
Collection date 08/24/2021
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations

Was found in my moms rose bush
Putative identification Arthropoda Hexapoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The wolbachia positive controls were the only ones that showed in the electrophoresis process for Wspec
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
GTTCTTTAATTGGAGATGATCAAATTTATAATGTTATTGTTACAGCTCACGCTTTTATTATAATTTTTTTTATAGTTATA CCCATTATAATTGGTGGATTTGGTAATTGATTAGTACCTTTAATATTAGGAGCCCCAGATATAGCTTTTCCACGAATAAA TAATATAAGATTTTGACTTCTCCCACCCTCAATTTTATTATTATTAATTAGAAGAACAGTAGAAAGAGGAGCAGGAACTG GATGAACTGTATACCCCCCTTTATCAGCAAGAATTGCTCATGCAGGACCAGCAGTAGATTTAACAATTTTTTCTCTTCAT CTTGCAGGTATATCTAGAATTATAGGTGCTGTAAACTTTATTACAACTATAATTAATATAAAACCACTTTATATAAATCA AACCCAAGTACCTCTTTTTGTATGATCAGTAGGAATTACAGCTCTATTATTATTACTTTCATTACCAGTACTTGCAGGAG CAATTACTATACTCTTAACAGATCGAAATCTTAATACTTCATTCTTTGATC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hexapoda was found to be negative for Wolbachia.
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