Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/19/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | The School for Science and Math at Vanderbilt |
| Observations | Turfgrass Ant with an unusually large gaster and clear wings. The coloration is brown and honey colored with a pattern resembling stripes on the gaster. Anatomy of ant is otherwise typical. Found crawling on pavement next to Rudbeckia hirta flowers in the late afternoon. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The PCR DNA analysis step was redone by the Bordenstein lab due to thermocycler malfunctions. Other than that the experiment went according to protocol. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I have a high confidence level about the results of the wolbachia experiment testing. There is little to no contamination and the sequencing results are of high quality. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
TGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence | Download FASTA
Download AB1
AATCGGTTCATCTATAAGAATAATTATTCGTCTAGAATTAGGATCATCTAATTCATTGATTAACAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCATTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTCCTTGTACCTTTAATATTAGGCTCACCTGATATAGCTTATCCACGTATAAACAATATGAGATTTTGACTTTTACCCCCCTCAATTTCTCTTCTTATTTTAAGAAATTTTATTAATGATGGTGTTGGGACAGGATGAACTGTTTATCCTCCTTTAGCTTCAAATATTTTCCATAATGGACCTTCAGTTGATTTAACTATTTTTTCCCTCCATATTGCGGGTATATCTTCAATTTTAGGAGCTATTAATTTTATTTCAACTATCTTAAATATACATCACAAAAATTTTTCTATCGATAAAATCCCTTTACTTGTATGATCAATTTTAATTACTGCAATTTTACTTCTCTTATCCTTACCCGTTCTTGCAGGAGCTATTACTATACTTTTAACTGACCGTAATCTTAACACTTCATTTTTTGATCCATCAGGTGGTGGAGATCCTATTTTATATCAACATCTTTTTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Lasius was found to be postive for Wolbachia. |
Water Boatman