Queen Turfgrass Ant – (Lasius Neoniger)

September 1, 2021

Sample information
Picture
Photos by: Ava T.
Location
Collection date 08/19/2021
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations

Turfgrass Ant with an unusually large gaster  and clear wings. The coloration is brown and honey colored with a pattern resembling stripes on the gaster. Anatomy of ant is otherwise typical. Found crawling on pavement next to Rudbeckia hirta flowers in the late afternoon.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Lasius

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The PCR DNA analysis step was redone by the Bordenstein lab due to thermocycler malfunctions.

Other than that the experiment went according to protocol.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I have a high confidence level about the results of the wolbachia experiment testing. There is little to no contamination and the sequencing results are of high quality.
Wolbachia 16S sequence Download FASTA    Download AB1
TGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
AATCGGTTCATCTATAAGAATAATTATTCGTCTAGAATTAGGATCATCTAATTCATTGATTAACAATGATCAAATTTATAATTCTATAGTTACAAGACATGCATTCATTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTTGGAAATTTCCTTGTACCTTTAATATTAGGCTCACCTGATATAGCTTATCCACGTATAAACAATATGAGATTTTGACTTTTACCCCCCTCAATTTCTCTTCTTATTTTAAGAAATTTTATTAATGATGGTGTTGGGACAGGATGAACTGTTTATCCTCCTTTAGCTTCAAATATTTTCCATAATGGACCTTCAGTTGATTTAACTATTTTTTCCCTCCATATTGCGGGTATATCTTCAATTTTAGGAGCTATTAATTTTATTTCAACTATCTTAAATATACATCACAAAAATTTTTCTATCGATAAAATCCCTTTACTTGTATGATCAATTTTAATTACTGCAATTTTACTTCTCTTATCCTTACCCGTTCTTGCAGGAGCTATTACTATACTTTTAACTGACCGTAATCTTAACACTTCATTTTTTGATCCATCAGGTGGTGGAGATCCTATTTTATATCAACATCTTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lasius was found to be postive for Wolbachia.
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