PG1 Arthropod

September 18, 2026

Sample information
Picture
Entry by: Prachi G
Location
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Oak Ridge High School
Observations

Wings, small body, long legs, big eyes
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am very confident because the controls were accurate proving I had no cross contamination and can trust that the arthropod did not have Wolbachia. There was a band for the Arthropod CO1 gene but not for the Wolbachia 16S gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
Report Inappropriate Post