Pillbug

September 18, 2026

Sample information
Picture
Entry by: Elizabeth H
Location
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Oak Ridge High School
Observations

The arthropod was inhabiting the underside of a block of concrete, located in a shaded and damp area near a wetland.
Putative identification Arthropoda Malacostraca Isopoda Armadillidae Armadillidium Armadillidium vulgare

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit.
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am confident in my results because both of the controls worked.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidium vulgare was found to be negative for Wolbachia.
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