Common House Mosquito Larvae

May 29, 2020

Sample information
Picture
Location
Collection date 05/30/2020
Captive / Cultivated? Wild-caught
Group
Observations

Sample 93
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex Culex pipiens

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
AGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGTGCGCAAGCCTAAGCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTTTGGGGCTTGAGCTGGAATAGTTGGAACTTCTTTAAGTTTACTAATTCGAGCAGAATTAAGTCAACCAGGTGTATTTATTGGAAATGATCAAATTTATAATGTTATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTAATACCAATCATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATGTTAGGAGCTCCAGATATGGCCTTTCCTCGAATAAATAATATAAGTTTTTGAATACTACCTCCTTCATTGACACTACTACTTTCAAGTAGTTTAGTAGAAAATGGGGCTGGGACTGGATGAACAGTGTATCCCCCTCTTTCATCTGGAACAGCTCATGCTGGAGCTTCAGTAGACTTAGCTATTTTTTCTTTACATTTAGCAGGAATTTCATCAATTTTAGGTGCAGTAAATTTTATTACAACAGTAATTAATATACGATCTTCAGGAATTACTCTTGATCGAATACCTTTATTTGTTTGATCAGTAGTAATTACTGCAGTTTTATTACTTCTTTCTTTACCTGTTTTAGCTGGTGCTATTACTATGTTATTAACAGATCGAAATTTAAATACTTCATTCTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Culex pipiens was found to be postive for Wolbachia.
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