Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/02/2020 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Innovation Academy |
| Observations | Beetle samples from a culture from Josh’s Frogs herp site. trying to establish a working culture for further research. Gel Pic Below: Lanes 2-5 are T. confusum beetles. Lane 1 is positive W control and lane 9 negative W control. |
| Putative identification | Arthropoda Hexapoda Insecta |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Wspec primers. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Insecta was found to be postive for Wolbachia. |
Water Boatman