Tribolium confusum (confused flour beetles)

June 26, 2020

Sample information
Picture
Location
Collection date 06/02/2020
Captive / Cultivated? Captive / Cultivated
Group Innovation Academy
Observations

Beetle samples from a culture from Josh’s Frogs herp site. trying to establish a working culture for further research.

Gel Pic Below: Lanes 2-5 are T. confusum beetles. Lane 1 is positive W control and lane 9 negative W control.
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Wspec primers.

Results
Wolbachia presence Yes
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be postive for Wolbachia.
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