Sample information |
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| Picture |
|
|---|---|
| Location | Approximate location: 36.01, -84.27 |
| Collection date | 09/08/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Oak Ridge High School |
| Observations | It was found in a corner outside between brick walls, and it was caught off of its orb web. |
| Putative identification | Arthropoda Arachnida Araneae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | By the end of gel electrophoresis, a pipetting error left half the expected solution behind to be sent out for testing. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | The QS results for Wolbachia from the sample were incredibly low, but I am unsure of their validity due to the volume of solution we sent in for this arthropod. |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download AB1
|
| Summary | The Araneae was found to be postive for Wolbachia. |
ABRHS 2026 – Ant – 12-3 Synemosyna Formica
ZETNJM Hymenoptera
CON-SCA Hemiptera
CONDJ Hemiptera