Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 36.00, -84.16 |
| Collection date | 09/09/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Oak Ridge High School |
| Observations | Found in a corner outside between brick walls, caught off of its orb web. |
| Putative identification | Arthropoda Arachnida Araneae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | Our QS results for the Wolbachia results was very low |
| Wolbachia 16S sequence | Download AB1
|
| Arthropod COI sequence | Download AB1
|
| Summary | The Araneae was found to be postive for Wolbachia. |
Ant
Wasp?
Castleback Orb Weaver