Diurnal Wasp

Sample information

Picture
Entry by: Isabella B
Location
Approximate location: 36.01, -84.27
Collection date 09/08/2026
Captive / Cultivated? Wild-caught
Group Oak Ridge High School
Observations

It was found on a brick wall outside of school in the morning, and it was about the size of a grain of rice. It shriveled up into a ball after freezing and treating with alcohol, leading to difficulties trying to identify its species. It was later identified as a wasp due to DNA test results.

Putative identification Arthropoda

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked properly during the gel electrophoresis process, highly decreasing the likelihood of contamination and false positives. If there had been false positives they likely would have been caused by cross contamination, which was not observable in the negative control, so we can infer that there was no cross contamination between samples throughout the process of PCR.

Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Arthropoda was found to be negative for Wolbachia.
Report Inappropriate Post