Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 36.01, -84.27 |
| Collection date | 09/08/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Oak Ridge High School |
| Observations |
|
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | 1X TBE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls worked properly during the gel electrophoresis process, highly decreasing the likelihood of contamination and false positives. If there had been false positives they likely would have been caused by cross contamination, which was not observable in the negative control, so we can infer that there was no cross contamination between samples throughout the process of PCR. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |

ABRHS 2026 – Ant – 12-3 Synemosyna Formica
ZETNJM Hymenoptera
CON-SCA Hemiptera
CONDJ Hemiptera