Wasp?

October 14, 2026

Sample information
Picture
Entry by: ISB
Location
Approximate location: 36.01, -84.27
Collection date 09/09/2026
Captive / Cultivated? Wild-caught
Group Oak Ridge High School
Observations

Found on a brick wall outside of school in the morning, about the size of a grain of rice. Shriveled up into a ball after freezing and treating with alcohol…
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked properly during the gel electrophoresis process, highly decreasing the likely hood of contamination and false positives.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Arthropoda was found to be negative for Wolbachia.
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