Plant Hopper – BCC Wolbachia Lab

October 13, 2026

Sample information
Picture
Entry by: Daniel S
Location
Approximate location: 42.44, -73.26
Collection date 10/06/2026
Captive / Cultivated? Wild-caught
Group Berkshire Community College
Observations

Arthropod was found on the bark of a tree along the sidewalk in an urban environment.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit Monarch DNA extraction (NEB)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes
  1. We used a DNA extraction protocol based on the insect adaptation of New England Biolabs’ Monarch Spin gDNA Extraction kit (Product #T3010)
  2. The specimen was incubated for 45 minutes in a hot water bath at 56 degrees C.

Our first PCR was set up on October 21st 2026. It was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16S primers together. It used an annealing temperature of 49 degrees Celsius. It also used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#M0488S).

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
Report Inappropriate Post