Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/18/2021 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | The School for Science and Math at Vanderbilt |
| Observations | post-rain, evening, 73 degrees Fahrenheit, humid |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Typical standard operating procedures |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Sequencing and gel electrophoresis both displayed wolbachia infection. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Orthoptera was found to be postive for Wolbachia. |
Water Boatman