Katydid

September 7, 2023

Sample information
Picture
Photos by: Gabby H.
Location
Collection date 08/18/2021
Captive / Cultivated? Captive / Cultivated
Group The School for Science and Math at Vanderbilt
Observations

post-rain, evening, 73 degrees Fahrenheit, humid
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Typical standard operating procedures

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Sequencing and gel electrophoresis both displayed wolbachia infection.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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