Sample information

Photos by: Chelsea Erwin
Collection date 08/08/2021
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

4 pairs of legs, 8 in total, with 2 body segments. Brown body with translucent legs and brown knobs for joints. The body is fuzzy.

Putative identification Arthropoda Chelicerata Arachnida Araneae Araneidae


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

We had to redo the second arthropod gel due to a mistake made with the gel at first, and may have gone too roughly with pulling it out (which can be seen with the less visible wells in the second gel). Complications with cross-contamination may have occurred, with (more or less confident) false negatives in 01-A, 06-A and the Wolbachia-Arthropod DNA which should all have had positive bands, however 01-A and 06-A could have been done correctly.


Wolbachia presence No
Confidence level High
Explanation of confidence level

Wolbachia is not as commonly found in spiders, and with how it had no response to either gel makes sense. Spiders also don’t commonly have arthropod DNA, and thereby wouldn’t have had a positive result in the arthropod gel. Due to this, in both gels, where the spider tested negative, it gives high confidence that the experiment was done correctly.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneidae was found to be negative for Wolbachia.
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