Common Cicada

October 15, 2026

Sample information
Picture
Entry by: Caleb Johnson
Location
Approximate location: 36.02, -84.26
Collection date 09/08/2026
Captive / Cultivated? Wild-caught
Group Oak Ridge High School
Observations

I caught the cicada in a wetland.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

Followed protocol exactly.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Very confident due to following protocol correctly.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Arthropoda was found to be postive for Wolbachia.
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