Pale Green Assassin Bug 2026

October 15, 2026

Sample information
Picture
Entry by: Izzy S.
Location
Approximate location: 36.01, -84.27
Collection date 09/09/2026
Captive / Cultivated? Wild-caught
Group Oak Ridge High School
Observations

I captured my arthropod underneath of a zinnia leaf in our garden.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

I followed protocol exactly according to the instructions given.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The line on my arthropod’s Electrophoresis was clear and the controls were accurate too.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
Summary The Hemiptera was found to be negative for Wolbachia.
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