Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 39.30, -77.65 |
| Collection date | 09/05/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Captured in grassy lawn, at the edge of the woods. Captured in a paper cup, moved to a plastic container, put in freezer, relocated to different freezer ~2h, covered in isopropanol. Season: Late Summer Temperature: 80 F Habitat: Terrestrial Brown, no wings present, 8 legs, unclear eye shape, external mandible for chewing, non-modified fore-legs or hind-legs, no antenna, no apparent body regions, laterally compressed body |
| Putative identification | Arthropoda Arachnida Opiliones Sclerosomatidae Leiobunum Leiobunum vittatum |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | DNA Extraction Centrifuge briefly set to 10,000 rpm instead of 8,000 rpm for the first spin PCR Spilled a small amount of water on the table Gel Electrophoresis D3 sample (not this one) did not show positive; issue was unique to D3 in DNA Extraction lab
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Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The protocol for this sample went on without any visible mistakes — we have a high confidence level in our gel results because positive and negative Wolbachia and Arthropod controls were included and the positive Arthropod and negative Wolbachia controls showed similar results in the gel |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
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| Summary | The Leiobunum vittatum was found to be negative for Wolbachia. |
CON-JRR Neuroptera
CON-RXB Hymenoptera
CONMED Diptera
CON-MAR Hemiptera
CON-CBS Blattodea