DEM-AHR Diptera

November 2, 2026

Sample information
Picture
Entry by: AniaR
Location
Approximate location: 31.97, -81.16
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The collection method I used was a sweep net.

Managed Grassland

Collected ants, a few love bugs, a gnat, and a unknown bug.

Collected on the side of Jenkins hall in front of the bushes and in front of Jenkins hall on the grass on the side of the sidewalk
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No band was shown for my insect gel electrophoresis results.
Wolbachia 16S sequence
Arthropod COI sequence
CCTGATATAGCTTTTNNTCGAATAAATAATAT ANNNTTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTACTGGATGAA CAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTCTTCATTTAGCT GGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACATTTGATCG TATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTAGCCGGGGCTATTA CTATACTATTAACAGATCGAAATTTAAATACAAGTTTTCTTTGACCCAGCAGGAGGGGGAGACCCTATTTTATACCAACA TTTATTTTGANTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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