Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 08/31/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Sweep Net grassland |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am confident my insect is not infected with Wolbachia, however the order I predicted was wrong. I trust the controls and result. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GAACNTCNCTAANTATACTTATTCGAGTAGAACTGGGAAAT CCAGGAACCTTTATTGGAAATGATCAAATTTATAATTNAATCGTAACGGCCCACGCTTTTATTATAATTTTCTTCATAGT TATACCAATTATAATTGGAGGTTTTGGTAACTGACTAGTACCCCTTATATTAGGTGCCCCTGATATAGCATTCCCCCGAA TAAATAATATAAGATTTTGATTACTACCCCCTTCNTTGAATCTACTTATTATAAGAAGAATTGTANAAAGTGGGGTAGGA ACTGGATGAACAGTTTATCCCCCTCTATCNTCTAATATTGCTCATAGGGGGNCNTCTGTANACCTTGCCATTTTNNNATT ANNTTTAGNAGGNATTTCNTCNATTTTAGGTGCTGTAANTTTTCATTTNNNCAGTTATTAATATACGACCACNANGTATA TTTATAGANCNAACACCNTTATTTGTATGA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Westhoney bee
(no title)
DEMLJS