Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 31.98, -81.16 |
| Collection date | 08/31/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Caught with gloved hands on leaves. Located near a garden. |
| Putative identification | Arthropoda Insecta Hemiptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The positive, negative, and NTC controls performed correctly. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATATTAATTCGTGTTGAATTANCCCAA CCTGGTTCTTTTTTTNNNAATGATCAAATATACAATGTAATTGTTACNTCNCATGCTTTTATTATGATTTTTTTTATAGT TATACCTATTATAATTGGTGGGTTTGGAAATTGATTACTACCTTTGATAATTGGTGCTCCTGATATGGCTTTTCCACGAC TAAATAATATAANATTTTGATTATTANTTCCATCTTTATCTTTATTAATTTTAANTTCTTTTGTTGAANTAGGGGCTGGA ACTGGTTGAACTGTCTACCCACCTCTTTCNTCTAATATTGCTCANNCAGGNGCTAGTGTANATTTANCTATTTTTTCNNN ACNTCNTGCCGGNATTTCTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
DEC-JAM Order Hemiptera
DECANA Hemiptera
(no title)
DECKNM Blattodea
DEM-RPW Odonata