DEC-JAM Order Hemiptera

November 2, 2026

Sample information
Picture
Entry by: Jacob M
Location
Approximate location: 31.98, -81.16
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected with a sweep net, found in tall grass next to a parking lot.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked, and there were no errors that I or my partner caught during the experiment.
Wolbachia 16S sequence
Arthropod COI sequence
AGACTAATTATCCGAATCGAACTA GGACAACCAGGAAGATTCATTGGAGATGATCAAATTTATAACGTAATCGTAACAGCTCACGCCTTCGTAATAATTTTCTT CATAGTAATACCTATTATAATTGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGCTCCCGACATAGCATTCC CTCGTATAAATAACATAAGATTTTGATTATTACCTCCTTCATTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGA GCTGGAACCGGATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCTCACAGAGGAGCATCCGTAGACTTAGCAATTTT TTCACTCCACTTAGCCGGGGTATCTTCAATCTTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGACCCACGG GAATAATCCCTGAACGAATTCCTTTATTTGTATGATCAGTAGGGATTACTGCCTTACTTCTACTACTATCATTACCTGTA CTAGCAGGTGCTATTACAATATTATTAACTGATCGAAATTTTAACACATCATTTTTTGATCCCTCANGTGGAGGGGACCC TATTTTATACCAACATTTATTTTGATTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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