MAT-NZB

November 3, 2026

Sample information
Picture
Entry by: N.B
Location
Approximate location: 31.97, -81.16
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Very confident that my insect did not contain Wolbachia.
Wolbachia 16S sequence
TATATTAATTCGAACAGAATT AAGCCATCCAGGATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTT TTATAGTTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTT CCTCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGG GGCTGGTACTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTT TTTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACT GGAATTACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGT TTTAGCCGGGGCTATTACTATACTATTAACAGATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACC CTATTTTATACCAACATTTATTTTGATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GTGGCGTGCTGATCCACGATTACTAGCGatcccttcttcgtgCACTCGAGTTGCAGAGTACAATCCGAACTGAgaTggcTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGACGAACCGACCCTATCCCTTCGAATAGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
Report Inappropriate Post