Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 31.98, -81.16 |
| Collection date | 09/01/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Caught with gloved hands on a small landscape near the road in front of the UPD station.It was September 1, 2026. The time was around 10:21 a.m. The area already had many mosquitoes, and damp grass. For temperature it was hummid. |
| Putative identification | Arthropoda Insecta Hemiptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
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| Protocol notes | DNA extraction kit of in house reagents was used. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | My positive, negative, NTC controls, as well as my partner’s DNA, performed correctly. |
| Wolbachia 16S sequence |
GTGTACAGacCCGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGTCTTTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAGCCCATTGTAGACACCATTGTAGCACGTGTGTAGCCCACTTCATAAAGGCCATGATGACTTGACATCATCCCCATCTTCCTCCAGCTTATCACTGGCAGTTTCCTTAAAGTGCTCAGCATTACCTGATGGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACAC=CTGTGTAAAATCCGGCCGAACCGACCCTATCCCTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence |
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| Summary | The Hemiptera was found to be postive for Wolbachia. |
BRI-AP Orthoptera
BRI AJC Diptera
CON-ACS Lepidoptera
CON-EJC Orpthoptera
CONKAE-Orthoptera