Diptera

November 3, 2026

Sample information
Picture
Entry by: BW
Location
Approximate location: 31.97, -81.16
Collection date 01/20/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Observed only 1 pairing of true wings for Diptera.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have a high confidence level that my insect sample is negative because there were no detections for Woibachia in my Diptera sample.
Wolbachia 16S sequence
Arthropod COI sequence
TAAGTATATTAATTCGAACAGAATTA AGCCATCCAGGATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTTT TATAGTTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTC CTCGAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGG GCTGGTACTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTT TTCTCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTG GAATTACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTT TTAGCCGGGGCTATTACTATACTATTAACAGATCGAAATTTAAATACNAGTTTCTTTGACCCAGCNNNGGGGGAGACCCT ATTTTATACCNACATTTATTTTGATTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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