MATSKB Diptera

November 3, 2026

Sample information
Picture
Entry by: Starr B
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

one pair of wings
Putative identification Arthropoda

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have high confidence in the negative result becuase no band was deteted at the expected wolbachia 16s . The presence of the CO1 band would confrim that the sample contained amplifiable DNA, supporting a true negative rather than a failed reaction
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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