DEM-RN Hemiptera

November 3, 2026

Sample information
Picture
Entry by: Raiti N
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Date: 9/1/2026

Time: 1:50 PM

Location: Behind the greenhouse on the Georgia Southern Armstrong Campus

Environment: Long grassy area next to water and a parking lot 8

Method of Collection: Collected via gloves hands individually capturing insects 
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All test/control PCR samples held predicted results, so it can be assumed that PCR methods used are reliable.
Wolbachia 16S sequence
Arthropod COI sequence
TATAGTAGGNATCCGCNNATNAGACTAATTATCCGAATCGAACTAGGACAACC AGGAAGATTCATTGGAGATGATCAAATTTATAACGTAATCGTAACAGCTCACGCCTTCGTAATAATTTTCTTCATAGTAA TACCTATTATAATTGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGCTCCCGACATAGCATTCCCTCGTATA AATAACATAAGATTTTGATTATTACCTCCTTCATTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGAGCTGGAAC CGGATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCTCACAGAGGAGCATCCGTAGACTTAGCAATTTTTTCACTCC ACTTAGCCGGAGTATCTTCAATCTTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGACCCGCAGGAATANTC CCTGAACGAATTCCTTTATTTGTATGANCAGTAGGGATTACTGCCTTACTTCTACTACTATCATTACCTGTGCTAGCAGG NGCTATTACAATATTATTAACTGATCGAAATTTTAACACATCNTTTTTCGATCCNTCNNTGGAGGAGAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
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