DEM-EAK Orthoptera

November 3, 2026

Sample information
Picture
Entry by: Elaina K
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Date of collection: 9/1/2026
  • Time of collection: 1:46pm
  • Location collection: Behind the greenhouse in front of the pond
  • Description of location: Very tall and thick grass, thorny areas, and big oak trees
  • Method of collection: I caught them all with my gloved hands because the D net was not working out
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes
  1. DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident that my insect does not have Wolbachia because it did not exhibit the band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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