CON-MKK Orthoptera

November 4, 2026

Sample information
Picture
Entry by: Moira King
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • From 6:00-6:30 PM 
  • Field and parking lot behind the Science Center
  • Tall grass, small tree, shallow puddle, underneath small log and wood pallet
  • D-net, sweep net, beat net and stick, 2 specimen collection jars, and forceps
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel band for Wolbachia within my sample is clearly absent from the gel electrophoresis results I conducted with my partner. Plus, I am confident in this result, as I predicted the same outcome at the beginning of the semester.
Wolbachia 16S sequence
Arthropod COI sequence
ATTATTAATTCGAGCT GAGCTAGGACAACCAGGTTTTCTTATTGGAGATGACCAAATTTATAACGTTATTGTAACGGCACATGCCTTTGTTATAAT TTTTTTTATAGTTATACCAATTATAATCGGAGGATTCGGAAATTGGTTAGTTCCTTTAATATTGGGGGCTCCAGATATAG CTTTCCCACGAATAAACAATATAAGCTTTTGACTTTTACCCCCATCCTTGACCTTACTTCTAGCCAGAAGTCTTGTCGAA AACGGGGCAGGTACTGGTTGAACAGTTTATCCACCACTTTCTGCTAATATTGCGCATGCAGGAGCCTCAGTTGATCTAGC TATCTTCTCATTACATTTAGCAGGTATTTCCTCAATTTTAGGGGCTGTAAATTTTATTACTACAACAATTAATATACGAG CCCCTGGAATATCCCTAGATCAAACCCCCTTATTTGTATGAGCAGTAGCTATCACAGCACTACTTTTATTATTATCTTTA CCAGTATTAGCAGGAGCTATTACTATATTATTAACCGATCGAAATTTAAATACATCCTTCTTTGACCCTGCTGGAGGTGG AGACCCTATTCTTTACCAACACTTATTCTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
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