November 4, 2026

Sample information
Picture
Entry by: Logan D
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Two pairs of wings, mandibles, different wing textures, abdomen missing pinchers, leathery and flexible forewings overlapping at rest, simple front legs, back legs modified for jumping.
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I had already done gel electrophoresis and was not confident in my sample having Wolbachia after having seen the occurrence rate in order Orthoptera.
Wolbachia 16S sequence
Arthropod COI sequence
AATAATTATTCGAGCA GAATTAGGTCAACCAGGATCCCTAATTGGAGATGACCAAATTTATAATGTAATTATTACAGCCCATGCATTTGTAATAAT TTTCTTTATAGTTATACCAATTATAATTGGAGGATTTGGTAACTGACTTGTACCATTAATAATTGGAGCACCAGATATAG CATTTCCACGAATAAATAACATAAGTTTTTGACTTTTACCACCATCATTAACCCTACTACTTACATCCTCTATAGTGGAT AATGGAGCTGGGACAGGATGAACAGTTTACCCCCCACTTGCTGGTGCAATTGCTCACGGAGGAGCATCGGTTGATTTAGC TATTTTCTCTCTCCATTTAGCTGGTGTATCATCAATTCTAGGAGCAGTTAATTTTATTACAACAGCAATTAATATACGAT CAGAAAGTATAACATTAGATCAAACACCACTATTTGTATGATCAGTTGCTATTACAGCCCTTCTTTTATTATTATCACTT CCTGTACTAGCTGGAGCTATTACAATATTATTAACTGATCGAAACCTAAATACATCATTTTTTGACCCCGCANGANGGGG TGACCCAATTCTTTATCNACACTTATTTTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be negative for Wolbachia.
Report Inappropriate Post