Eastern Leaf Footed Bug

October 6, 2021

Sample information
Picture
Photos by: Emiliano A.
Location
Collection date 09/24/2021
Captive / Cultivated? Wild-caught
Group The School for Science and Math at Vanderbilt
Observations

Found on tomatillo plants in a colony.
Putative identification Arthropoda Hexapoda Insecta Hemiptera Coreidae Leptoglossus Leptoglossus phyllopus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The Bordenstein Lab had to rerun some of our samples, since the PCR machine failed while running our DNA.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked great along with the other samples. I also had an accurate identification with the Co1F.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
AGTAGGTTCATCTATAAGATGAATTATCCGAGTAGAACTAGGTCAACCAGGTTCATTTATTGGTGATGATCAAATTTATA ATGTAATTGTAACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCAATTATAATTGGTGGGTTTGGAAACTGA TTAGTCCCTTTAATAATTGGAGCCCCTGATATAGCTTTCCCTCGTATAAATAATATAAGTTTTTGATTACTACCACCTTC TTTAACCCTTTTATTAACAAGCAGAATAGTAGAAATAGGTGCAGGTACAGGTTGAACTGTTTATCCGCCCCTATCTAGTA ATCTTTCTCATAGAGGAGCATCTGTTGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTTTCATCAATTCTAGGAGCA GTAAATTTTATTTCAACAATTATAAACATACGACCCACTGGAATAACCCCTGAACGGACCCCATTATTTGTATGATCTGT TGGCATTACAGCATTACTCTTACTCCTTTCACTTCCAGTTCTTGCAGGAGCCATCACTATACTTTTAACAGACCGAAATT TTAATACATCTTTCTTTGACCCTACTGGAGGAGGGGATCCAATTTTGTATCAACACTTATTCTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Leptoglossus phyllopus was found to be negative for Wolbachia.
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