Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/24/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | The School for Science and Math at Vanderbilt |
| Observations | Found on tomatillo plants in a colony. |
| Putative identification | Arthropoda Insecta Hemiptera Coreidae Leptoglossus Leptoglossus phyllopus |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | The Bordenstein Lab had to rerun some of our samples, since the PCR machine failed while running our DNA. |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The controls worked great along with the other samples. I also had an accurate identification with the Co1F. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
AGTAGGTTCATCTATAAGATGAATTATCCGAGTAGAACTAGGTCAACCAGGTTCATTTATTGGTGATGATCAAATTTATA ATGTAATTGTAACAGCTCATGCTTTTATTATAATTTTCTTTATAGTTATACCAATTATAATTGGTGGGTTTGGAAACTGA TTAGTCCCTTTAATAATTGGAGCCCCTGATATAGCTTTCCCTCGTATAAATAATATAAGTTTTTGATTACTACCACCTTC TTTAACCCTTTTATTAACAAGCAGAATAGTAGAAATAGGTGCAGGTACAGGTTGAACTGTTTATCCGCCCCTATCTAGTA ATCTTTCTCATAGAGGAGCATCTGTTGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTTTCATCAATTCTAGGAGCA GTAAATTTTATTTCAACAATTATAAACATACGACCCACTGGAATAACCCCTGAACGGACCCCATTATTTGTATGATCTGT TGGCATTACAGCATTACTCTTACTCCTTTCACTTCCAGTTCTTGCAGGAGCCATCACTATACTTTTAACAGACCGAAATT TTAATACATCTTTCTTTGACCCTACTGGAGGAGGGGATCCAATTTTGTATCAACACTTATTCTG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Leptoglossus phyllopus was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid