Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 08/22/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | The School for Science and Math at Vanderbilt |
| Observations | Found in a wet and shady area, under a tree |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Tenebrionidae Heterotarsus Heterotarsus carinula |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Controls worked, electrophoresis successful, arthropod identified, insect identified as something else (all info here was changed). |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
TTTGCGGGANTGGTAGGTGGTGCTTTGTCTTTGATTTTTCGCATGCAACTTATGCATATCAACATCTTTGGTGATAATTATCAATTATATAATGTAGTAGTTACAGGCCATGCTTTGATTATGGTGTTTTTTATGATTATGCCAGCTTTGATGGGTGGTTTTGGTAATTGGTTTGTTCCCCTTATGATTGGTGCACCAGATATGGCTTTTCCACGTATGAATAACTTGAGCTTTTGGTTGCTTGTATCCTCGTTTATTTTACTTATTATGTCTGTATTTGTTGGTGAAGGAGCTGGTACTGGTTGGACCTTATACCCACCACTATCACAGGTTAACTCTCACCCTANTGCAGCTGTTGATTTAGCAATATTTGCAATTCACGTTGCTGGAATTTCCTCAATTGTCGGTTCTATTAATTTTATTGTTACTATATTTAATATGCNCACTCCTGGAATGACTTTACATAAAACGCCTTTATTTGTTTGGTCTATTTTACTTACAGCTTTTATGCTTATAATTGCTTTACCA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Heterotarsus carinula was found to be negative for Wolbachia. |
Vespidae Maculifrons
Tenebrio molitor
Wasp-Hymenoptera sp
Pollenia pediculata,
Phyciodes tharos