ZETNJM Hymenoptera

November 12, 2026

Sample information
Picture
Entry by: Neilani M.
Location
Approximate location: 31.97, -81.16
Collection date 08/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Collection method/equipment:

Detailed description of the location of the collection site:

Detailed description of the habitat of the collection site:

Detailed description of the various collected specimens:
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence is high because all controls worked as expected. The positive control showed bands for both arthropod COI and Wolbachia 16S, the negative control showed no contamination, and my sample showed a clear arthropod band with no Wolbachia band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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