Sample information
Picture	Entry by: Kaitlyn K
Location	Approximate location: 42.48, -71.46
Collection date	09/17/2026
Captive / Cultivated?	Wild-caught
Group	Acton-Boxborough Regional High School
Observations
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	There is a very clear band for the wolbachia gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be postive for Wolbachia.