Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/15/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Mosquito was resting on a hallway wall in my apartment building. It was very frantic for a while until it perished an hour before being placed in ethanol |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
CTGGAGTATTTATTGGAAATGATCAAATTTATAATGTTATTGTAACTGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTTCCTCTAATATTAGGAGCTCCTGATATAGCTTTTCCTCGAATAAATAATATAAGTTTTTGAATACTACCTCCTTCATTAACATTACTACTTTCAAGTAGTATAGTAGAAAATGGAGCTGGGACTGGATGAACAGTTTACCCCCCTCTTTCATCTGGTACAGCCCATGCTGGAGCTTCAGTAGATTTAGCTATTTTTTCATTACATTTAGCTGGAATTTCATCAATTTTAGGAGCAGTAAATTTTATTACTACTGTAATTAATATACGATCTTCAGGTATTACACTTGATCGAATACCATTATTTGTTTGATCAGTAGTAATTACTGCTGTTCTTTTACTTCTTTCTTTACCTGTATTAGCTGGTGCTATTACTATACTATTAACTGATCGAAATCTAAATACTTCGTTTTTTGACCCTATTGGA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Diptera was found to be negative for Wolbachia. |
Extraction and Analysis of Wolbachia from Halyomorpha halys
Tick
Roly-Poly
Idaho Beetle
Grammonota