Chrysoperla

November 3, 2021

Sample information
Picture
Photos by: Sascha H
Location No map location data available.
Collection date 09/23/2021
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations
Putative identification Arthropoda Hexapoda Insecta Neuroptera Chrysopidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All of our controls worked, and the lab which sequenced our DNA samples reported a high degree of confidence in their samples.
Wolbachia 16S sequence Download FASTA    Download AB1
NNNNNGNGGNNTTNNNNNTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCA ACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGTACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT GATGTCAAGTCATCATGGCCTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGTTGCAAGGTGCCCA AGCCTAACCTAATCCCTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTA ATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCNN NCGAAGCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
NNNNNNNANTTTCGGATTTGATCTGGATTAGTAGGTACAAGATTAAGTTTATTAATTCGAGCTGAATTAGGTCAACCAGG TTCTTTAATTGGTGATGATCAAATTTATAATGTTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATGGTAATAC CTATTGTTATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATATTAGCTGCTCCTGATATAGCATTTCCACGTATAAAT AATATAAGTTTTTGAATATTACCTCCTTCATTAACCTTATTACTTGCATCTTCAATAGTAGAAAGAGGAGCTGGGACTGG TTGAACAGTGTACCCCCCACTTTCTTCTAGAATTGCTCATGCTGGAGCTTCTGTTGATTTAGCTATTTTTAGTTTACATT TAGCTGGTATCTCTTCAATTTTAGGGGCTGTTAATTTTATTACAACAGTAATTAATATACGATTAAGTTATATAACTTTA GATCGAATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTATTACTATCTTTACCTGTTTTAGCTGGAGC TATTACCATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCTGGAGGAGGAGATCCAATTTTATACCAACATTTATTTTGATTTTTTGNNNNCNNGNNNNNANNNNNNNN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Chrysopidae was found to be postive for Wolbachia.
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