Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/10/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Acton-Boxborough Regional High School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Carabidae Harpalus Harpalus rufipes |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TAE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Harpalus rufipes was found to be negative for Wolbachia. |
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus