Sample information
Picture	Photos by: Seanna Lee
Location
Collection date	09/10/2021
Captive / Cultivated?	Wild-caught
Group	Acton-Boxborough Regional High School
Observations
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Carabidae Harpalus Harpalus rufipes
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	TAE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Harpalus rufipes was found to be negative for Wolbachia.