K.4.2 fly

Sample information

Photos by: Ethan Z., Legend Y., Gavin K.
Collection date 02/23/2022
Captive / Cultivated? Wild-caught
Group Singapore American School
  • Wings
  • Black body
Putative identification Arthropoda Hexapoda Insecta Diptera


Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer PBS
DNA stain GelGreen
Gel images
Protocol notes


Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Controls worked, but absence in the sample may not be evident of absence in the whole organism

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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