Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/23/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Singapore American School |
| Observations |
|
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | PBS |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Controls worked, but absence in the sample may not be evident of absence in the whole organism |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman