Angelo’s Wolbachia Project

March 30, 2022

Sample information
Picture
Photos by: Angelo C.
Location
Collection date 09/14/2021
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

Very tiny
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Duplex PCR was performed.

Edvotek was used for PCR

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

My confidence level is low because one of the controls needed to confirm whether or not my arthropod had Wolbachia was not present, therefore, no confidence and exact conclusion can be made.
Wolbachia 16S sequence
Primers used were from Wolbachia Project
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Primers used were from Wolbachia Project
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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