Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/14/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | Very tiny |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Duplex PCR was performed. Edvotek was used for PCR |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My confidence level is low because one of the controls needed to confirm whether or not my arthropod had Wolbachia was not present, therefore, no confidence and exact conclusion can be made. |
| Wolbachia 16S sequence |
Primers used were from Wolbachia Project
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
Primers used were from Wolbachia Project
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
Water Boatman