Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/14/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | The insect is white with one black spot on each wing. About an inch to an inch and a half total wingspan. |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera Pieridae Pieris Pieris rapae |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Duplex PCR reaction was used. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My controls had errors, namely a 708 bp band in the negative control lane (water), and no band in the negative arthropod control (which should have had a 708bp band). |
| Wolbachia 16S sequence |
Provided by Wolbachia Project
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
Provided by Wolbachia Project
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | |
Water Boatman