Charlotte’s Wolbachia Results 3/31/22

March 31, 2022

Sample information
Picture
Photos by: Charlotte D.
Location
Collection date 09/14/2021
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

The insect is white with one black spot on each wing. About an inch to an inch and a half total wingspan.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera Pieridae Pieris Pieris rapae

Methods
Extraction kit Edwards Buffer
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Duplex PCR reaction was used.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

My controls had errors, namely a 708 bp band in the negative control lane (water), and no band in the negative arthropod control (which should have had a 708bp band).
Wolbachia 16S sequence
Provided by Wolbachia Project
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Provided by Wolbachia Project
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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