Common Pill bug

April 4, 2022

Sample information
Picture
Location
Collection date 03/31/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

Moves quickly

Rolls easily

1cm long

0.3cm wide

Rolly Polly!
Putative identification Arthropoda Crustacea Malacostraca Isopoda

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We made an error in pipetting into well 4.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Our results were inclusive. Neither of our PCRs passed through.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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