Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/31/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | Moves quickly Rolls easily 1cm long 0.3cm wide Rolly Polly! |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We made an error in pipetting into well 4. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Our results were inclusive. Neither of our PCRs passed through. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Millipede
Cylindroiulus caeruleocinctus
Biting Midge
Eastern Red Centipede
Specimen_3