Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Richmond High School |
| Observations | The black fly had 8 legs, big eyes, and a noticeable head, thorax, abdomen, with wings. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Stratiomyidae Hermetia Hermetia illucens |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | It was very straight forward and very simple to do but Wolbachia is not present |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I feel confident as I didn’t think it would be positive |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hermetia illucens was found to be negative for Wolbachia. |
Water Boatman