Black fly

April 4, 2022

Sample information
Picture
Photos by: Molly O'Brien
Location
Collection date
Captive / Cultivated? Captive / Cultivated
Group Richmond High School
Observations

The black fly had 8 legs, big eyes, and a noticeable head, thorax, abdomen, with wings.
Putative identification Arthropoda Hexapoda Insecta Diptera Stratiomyidae Hermetia Hermetia illucens

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

It was very straight forward and very simple to do but Wolbachia is not present

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I feel confident as I didn’t think it would be positive
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hermetia illucens was found to be negative for Wolbachia.
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