Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/01/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | A tiny mite that looks like a crab |
| Putative identification | Arthropoda Chelicerata Arachnida |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Other |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | My confidence in my level is pretty low due to the wells not being deep enought for the liquids |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata