Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | Black beetle, small with lines on the back. |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Carabidae Pterostichus |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | My DNA for my bug was the only one that showed on the gel. My bug was negative for Wolbachia. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I know that the common black ground beetle i found was negative for Wolbachia because i got the DNA but the wolbachia slot was negative. I’m confident because we followed the protocol exactly. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Pterostichus was found to be negative for Wolbachia. |
Water Boatman