|Captive / Cultivated?||Wild-caught|
|Group||Richmond High School|
Black beetle, small with lines on the back.
|Putative identification||Arthropoda Hexapoda Insecta Coleoptera Carabidae Pterostichus|
|Extraction kit||Thermoscientific GeneJet Genomic Purification|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Duplex Reaction|
|Gel electrophoresis system||Edvotek Gel Electrophoresis|
|DNA stain||SYBR Safe|
My DNA for my bug was the only one that showed on the gel. My bug was negative for Wolbachia.
|Explanation of confidence level||
I know that the common black ground beetle i found was negative for Wolbachia because i got the DNA but the wolbachia slot was negative. I’m confident because we followed the protocol exactly.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Pterostichus was found to be negative for Wolbachia.|