Mosquito Results

April 13, 2022

Sample information
Picture
Location
Collection date 03/07/2022
Captive / Cultivated? Wild-caught
Group River Valley Community College
Observations

Was found on a clay pot outside of building, multiple mosquitos were in the area.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Followed standard protocol.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The controls failed, so it is very difficult to say with one hundred percent certainty that it is either negative or positive.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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