Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/07/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | River Valley Community College |
| Observations | Was found on a clay pot outside of building, multiple mosquitos were in the area. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | Followed standard protocol. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The controls failed, so it is very difficult to say with one hundred percent certainty that it is either negative or positive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Extraction and Analysis of Wolbachia from Halyomorpha halys
Tick
Roly-Poly
Idaho Beetle
Grammonota