Blow Fly

April 13, 2022

Sample information
Picture
Photos by: Sawyer J.
Location
Collection date 03/23/2022
Captive / Cultivated? Wild-caught
Group River Valley Community College
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Calliphoridae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The arthropod control for the blowfly came back negative, which showed an error with one of the processes over the course of the project.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Calliphoridae was found to be negative for Wolbachia.
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