Sample information |
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Picture |
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Location | |
Collection date | 03/31/2022 |
Captive / Cultivated? | Captive / Cultivated |
Group | Richmond High School |
Observations | green, near waters little chilly, around trees and under a wooden table. |
Putative identification | Arthropoda Hexapoda Insecta Diptera Culicidae |
Methods |
|
Extraction kit | Thermoscientific GeneJet Genomic Purification |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | Edvotek Gel Electrophoresis |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
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Protocol notes | During the DNA extraction step when adding AW 1 buffer to the solution, the solution turned pink |
Results |
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Wolbachia presence | Unknown |
Confidence level | Medium |
Explanation of confidence level | The procedure went well but when the solution turned it could have altered the results. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary |