mosquito

April 18, 2022

Sample information
Picture
Photos by: Dhwani B., Savanna B.
Location
Collection date 03/31/2022
Captive / Cultivated? Captive / Cultivated
Group Richmond High School
Observations

green, near waters little chilly, around trees and under a wooden table.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

During the DNA extraction step when adding AW 1 buffer to the solution, the solution turned pink

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

The procedure went well but when the solution turned it could have altered the results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
Report Inappropriate Post