Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/12/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | Black, under a rock, and near dirt. |
| Putative identification | Arthropoda Myriapoda Diplopoda |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | N/A |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | I am very confident as the gel lab we did was inconclusive since no lines appeared for the arthropods. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman