Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | Yellow centipede, small, found under a rock with wet dirt, and a lot of legs, “scaly”, little antennas |
| Putative identification | Arthropoda Myriapoda Chilopoda |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | Confidence level would be medium because we think the testing got messed up and the positives and negative control did not show up in the gel when we put the black light on it |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman