Wolbachia

April 18, 2022

Sample information
Picture
Photos by: Hayden Scalf
Location
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

Black, in a tree, very small, Roly-Poly
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

There were no results for the Roly-Poly being wolbachia positive, but also no results for wolbachia negative
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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