Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 03/30/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | Black, in a tree, very small, Roly-Poly |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | There were no results for the Roly-Poly being wolbachia positive, but also no results for wolbachia negative |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Harmonia Axyridis (Asian Lady Beetle)
(no title)
Brown Marmorated Stink Bug Wolbachia Project
Asian Lady Beetle Wolbachia Project
Melanoplus sp.