Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/12/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | It looks health, it is black. |
| Putative identification | Arthropoda Myriapoda Chilopoda |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Maybe I accidentally mix with bubble in the process. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | I didn’t get any result. Some thing must went wrong. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Chilopoda was found to be negative for Wolbachia. |
Water Boatman