Centipede

April 27, 2022

Sample information
Picture
Photos by: Ishan H.
Location
Collection date 04/09/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

Long, skinny, 118 legs (59 on each side), two antennae, golden-brown/orange/yellow color, moves pretty fast, segmented body (2 legs per segment), back legs are elongated, a little shorter in length than pinky finger.
Putative identification Arthropoda Myriapoda Chilopoda Geophilomorpha

Methods
Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA may not have been extracted correctly, or arthropod may not have been crushed completely.

The DNA pellet was a tan-ish color at the bottom of the tube.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The negative control had Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Geophilomorpha was found to be negative for Wolbachia.
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