House Fly

April 27, 2022

Sample information
Picture
Location
Collection date 04/12/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy
Observations

2 wings

4 legs

Shiny black

brown beedy eyes

hair on the body

sliver glare on the skin

proboscis

was dead when found.

 
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit Edwards Buffer
DNA extraction location
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Both gels did not show. I think because it was all ready dead when founded.

Results
Wolbachia presence
Confidence level
Explanation of confidence level

Not confident because the – control showed for having wolbachia. Also the + control showed for not being an arthropod.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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