Leafhopper Wallbachia

Sample information

Photos by: Gokul P
Collection date 04/11/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

It is a small creature that is brown, has small legs, wings are large (located on its back), and has 2 black eyes. Also, it has a pair of wings, 3 pairs of legs, large head, feet look like they are spiky-ish, body is small, and wings are bigger than the head.

Putative identification Arthropoda Hexapoda Insecta Hemiptera


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

We had either contamination in our gels or incorrectly placed controls.


Wolbachia presence No
Confidence level Low
Explanation of confidence level

The negative control showed Wolbachia and the positive control didn’t show the Arthropod DNA.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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