Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/11/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | It is a small creature that is brown, has small legs, wings are large (located on its back), and has 2 black eyes. Also, it has a pair of wings, 3 pairs of legs, large head, feet look like they are spiky-ish, body is small, and wings are bigger than the head. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | We had either contamination in our gels or incorrectly placed controls. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | The negative control showed Wolbachia and the positive control didn’t show the Arthropod DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Water Boatman